Title:

Human Lymphoblastoid Interferon: Purification, Amino Acid Composition, and Amino-Terminal Sequence

Creator:

Zoon, K. C. (Kathryn C.)
Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Zur Nedden, Dorothy
Bridgen, Pamela J.
Smith, Mark E.
Miller, Dorothea M.

Date:

1980

Description:

This article was the result of collaboration between various members of Anfinsen's lab at the NIAMD with Hunkapiller and Hood at Caltech. In it, they addressed the difficult problem of determining the amino acid sequence of Human Lymphoblastoid Interferon (HuIFN-alpha). Earlier attempts to sequence the amino-terminal portion of this molecule using conventional methods failed due to insufficient quantities of interferon. Through this collaboration, Anfinsen's lab developed a microsequencer, a technology that allowed them to obtain amino acid sequence data on 10 to 20 picomoles of protein. Because of this, they were able to successfully determine the amino-terminal amino acid sequence of the major component of human lymphoblastoid interferon (HuIFN-alpha).

Periodical:

Annals of the New York Academy of Sciences

Publisher:

New York Academy of Sciences

Location:

Box: 28. Folder: 6

Rights:

Reproduced with permission of the New York Academy of Sciences. and http://www.nyas.org/

Genre:

Articles

Subject:

Interferons, Amino Acids, Amino Acid Sequence, and Lymphocytes

Format:

Text

Extent:

9 pages

Language:

English

Legacy Source Citation:

Periodical. Zoon, Kathryn C., Mark E. Smith, Pamela J. Bridgen, Dorothy Zur Nedden, Dorothea M. Miller, and Christian B. Anfinsen. "Human Lymphoblastoid Interferon: Purification, Amino Acid Composition, and Amino-Terminal Sequence." Annals of the New York Academy of Sciences 350, (1980): 390-398. Article. 9 Images.. Annals of the New York Academy of Sciences

Legacy ID:

KKBBLM

NLM ID:

101584571X177

Profiles Collection:

The Christian B. Anfinsen Papers

Shareable Link:

https://profiles.nlm.nih.gov/101584571X177